You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. HTRF certified . For more information about HTRF. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Plate cells Stimulate cells 3. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. 55 µL anti-cAMP-Cryptate. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. 41 mg/ml) was obtained from Thermo Fisher Scientific. The laser beam passes through the sample well. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. For more information. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Remove the plate sealer and read on an HTRF® compatible reader . FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. For more information about HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. Incubate overnight at RT. For a list of HTRF-compatible readers and set-up recommendations, please visit . For more. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Incubate 4h at RT. Live Viewer for cell-based assays. This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED READER. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Its. culture plate. It enables you to gain insights you couldn’t achieve with detection measurements alone. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. Read & analyse. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. The kit includes everything you need: reagents, buffers and a microplate. It can also be further streamlinedFOR HTRF CERTIFIED READER. • HTRF®-Certified Reader. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. Revvity. Seal the plate and. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Other technologies such as imaging. This protocol gives the cells viability and confluence to be monitored. + 4 µL HTRF ® pre-mixed antibo dies 2. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. No washing steps are required. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. The plate was covered and incubated for two hours at room temperature. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. + 4 µL HTRF pre-mixed antibodies. Read & analyse. After an overnight incubation, the plate is washed 3 times. VICTOR® Nivo™ multimode plate reader 2 | For research use only. Plate 25 μL of cells in. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. FOR HTRF CERTIFIED READER For more. • HTRF®-Certified Reader. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER For more. Even if not listed, some. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. FOR HTRF CERTIFIED. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. For more information about set-up and compatible HTRF ® readers,• HTRF®-Certified Reader. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Partnerships for delivering turn-key solutions. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. In addition, stacker. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. This protocol, HTS designed, allows miniaturization while. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. Step 1 Step 2 Step 3 Step 4 Step 5. Read & analyse. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Remove the plate sealer and read on an HTRF® compatible reader . Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Catalog No. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. FOR HTRF CERTIFIED READER For. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Revvity. Customer Transformation Hub. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. Make sure the setup for Eu 3+ Cryptate is used. Remove the plate seal and read on an HTRF compatible reader. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. For more information about HTRF. We would like to show you a description here but the site won’t allow us. For a list of HTRF-compatible readers and set-up recommendations, please visit . Manufacturer. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Incubate 2h at RT Transfer 16 µL supernatant. If not, our scientific team will help you choose the instrument that best suits your needs and budget. * For HTRF microplate recommendations, please visit . 6% for 1,408 wells and a Z’ value of 0. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. MATERIALS PROVIDED: KIT . For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. CLARIOstar is equipped with a specific optical device which enables the measurement of. Remove the plate seal and read on an HTRF compatible reader. FOR HTRF CERTIFIED READER. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). These plates can be read on all HTRF certified readers, thus making their implementation in. Read & analyse. HTRF reagent concentrations have been set for optimal assay performances. FOR HTRF CERTIFIED READER. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Choosing the right microplate reader ensures you’ll get an optimal readout. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. FOR HTRF CERTIFIED READER. Figure 1. For more information. The plate is set up for reading and images are acquired according to specified parameters. For more information about HTRF. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. S. • HTRF®-Certified Reader **. 4 mg/ml. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. As shown in Fig. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. FOR HTRF CERTIFIED. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. Microplate readers are only certified if they. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. For more information about HTRF ®• HTRF®-Certified Reader. Make sure the setup for Tb Cryptate is used. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. Plate 25 μL of cells in. 5. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. Plate 25 μL of cells in. The VICTOR Nivo is a HTRF certified microplate reader. Your lab might even have one already. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. + 4 µL HTRF ® pre-mixed antibodies . Read & analyse. The detection antibody is incubated for 1. CLARIOstar is equipped with a specific optical device which enables the measurement of. FOR HTRF CERTIFIED READER. Both Microplate Optimization and Read Height Adjustment were. Read & analyse. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. FOR HTRF CERTIFIED READER. + 4 µL HTRF pre-mixed antibodies. HTRF Microplate Readers. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. View Brochure. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . Results for HTRF Reader Control Kit with black and white microplates. FOR HTRF CERTIFIED READER. $352. Assay setup for a 384-well low-volume plate. + 4 µL HTRF pre-mixed antibodies . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 10 µL Standard or Sample. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. For more information about HTRF. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. See the Cytation 5 Operator’s Manual for more information and instructions. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. At that stage, the analyte is sandwiched between the 2 antibodies. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Read & analyse. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. Microplate readers EnVision ® Multimode Plate Reader VICTOR® Nivo™ EnSight® Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ® The EnVision ® multimode plate reader provides exceptional speed,. Customer Transformation Hub. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Plate Type and Read Area should be selected based on the microplate used and wells to be read. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. This protocol gives the cells viability and confluence to be monitored. Each well generally requires 50µL of lysis buffer 1X. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. Plate cells Stimulate cells 3. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. It features a modular design that allows users to add or change detection cartridges according to their needs. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. For more information about HTRF. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. This protocol, HTS designed, allows. Certified for use with Alpha, LANCE ® or DELFIA ®. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Cover the plate with a plate sealer. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Plate 25 μL of cells in. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. FOR HTRF CERTIFIED READER. PURCHASE SEPARATELY . Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. Read & analyse. Simply place the module into one of the five module bays and read the assay. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. FOR HTRF CERTIFIED READER For more information about HTRF. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 5. FOR HTRF CERTIFIED READER For. Figure 1: Principle of HTRF Cortisol competitive assay. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. . Make sure the setup for Tb Cryptate is used. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Its. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . 25,000 human Jurkat cells were plated in 96 well plates. DLReady™ certified luminometer plate readers. These plates can be read on all HTRF certified readers, thus making their implementation in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. EGFR protein (PV3872, 0. Make sure the setup for Eu 3+ Cryptate is used. Make sure the setup for Tb Cryptate is used. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). For more information about HTRF. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. Revvity. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Table 1. Incubate overnight at RT. 5 EXAMPLE OF MAP PLATE . Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Plate 25 μL of cells in. overnight at 4 C [Prostaglandin E2] pg/ml. Read & analyse. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. For more. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. In particular, these parameters should be entered as below. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED READER. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Read & analyse. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. VICTOR Nivo Multimode Plate Reader. no. Lyse cells Read on an HTRF compatible reader . The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). 47 seconds for a 1536-well plate. This work reports 25 small-molecule binders of the m 6 A-reader domain of YTHDC1. Read & analyse. Endpoint mode experiments measure each well of the microplate only once. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. The ratio for each well must be calculated individually. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. 27 / Pack of 100; Qty. Incubate 4h at RT. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. Read & analyse. Read & analyse. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. 5 µL. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED READER For. One plate carrier adapter is provided with the instrument. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Read & analyse. For more ® information about HTRF compatible readers and for set-up recommendations. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. PerkinElmer. Smallest and most affordable multimode plate reader in the industry. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. This protocol enables the cells' viability and confluence to be monitored.